File:A-p38-Substrate-Specific-MK2-EGFP-Translocation-Assay-for-Identification-and-Validation-of-New-p38-pone.0095641.s004.ogv

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A-p38-Substrate-Specific-MK2-EGFP-Translocation-Assay-for-Identification-and-Validation-of-New-p38-pone.0095641.s004.ogv ‎(Ogg Theora video file, length 10 s, 768 × 576 pixels, 447 kbps, file size: 570 KB)

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English: Visualization of MK2-EGFP translocation after activation of p38 MAPK in real time. U2OS cells stably expressing MK2-EGFP were monitored for 90 min by fluorescent imaging at 3 min time intervals. Upper section: Cells were either activated by addition of hyperosmotic media [left] or treated with inhibitor (SB203580 (10 µM) [right]. Lower section: Cells were either activated by addition of hyperosmotic media immediately followed by treatment with p38 MAPK inhibitor (SB203580; 10 µM) [left] or incubated with DMSO (0.1 mM) [right].
Date
Source Movie S1 from Anton R, Bauer S, Keck P, Laufer S, Rothbauer U (2014). "A p38 Substrate-Specific MK2-EGFP Translocation Assay for Identification and Validation of New p38 Inhibitors in Living Cells: A Comprising Alternative for Acquisition of Cellular p38 Inhibition Data". PLOS ONE. DOI:10.1371/journal.pone.0095641. PMID 24743242. PMC: 3838353.
Author Anton R, Bauer S, Keck P, Laufer S, Rothbauer U
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current08:21, 22 April 201410 s, 768 × 576 (570 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 191 kbps Completed 00:50, 16 August 2018 6.0 s
Streaming 480p (VP9) 192 kbps Completed 05:22, 17 December 2023 1.0 s
VP9 360P 103 kbps Completed 00:50, 16 August 2018 3.0 s
Streaming 360p (VP9) 104 kbps Completed 19:21, 17 December 2023 1.0 s
VP9 240P 59 kbps Completed 00:50, 16 August 2018 3.0 s
Streaming 240p (VP9) 60 kbps Completed 07:17, 9 December 2023 1.0 s
WebM 360P 342 kbps Completed 08:22, 22 April 2014 6.0 s
Streaming 144p (MJPEG) 952 kbps Completed 10:51, 29 October 2023 1.0 s

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