File:Chrom SephG-50.tif

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Chrom_SephG-50.tif ‎(720 × 540 pixels, file size: 78 KB, MIME type: image/tiff)

Captions

Captions

Chromatogram of plant cytosol of Arabidopsis thaliana

Summary[edit]

Description
English: Chromatogram showing the UV absorption profile of Arabidopsis cytosol separated on Sephadex G-50 Superfine. Gel volume: 500 mL; column length: 700 mm; column diameter: 30 mm; eluent flow rate: 12 mL / hr; fraction volume: 8.0 mL; number of fractions: 95; sample volume: 5 mL; separation temperature: 4 °C; elution buffer: 20 mM Tris-HCl, 1 mM NaN3; pH 8.0. Metal cofactors and associated proteins (MW ≥ 30 kDa) that eluted in the range of the void volume (120 to 140 mL) of this method were identified and quantified by ICP-MS or GF-AAS.
Date
Source Open Biochem J. 2008; 2: 44–48;doi: 10.2174/1874091X00802010044
Author Bernd Kastenholz
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current16:08, 17 October 2010Thumbnail for version as of 16:08, 17 October 2010720 × 540 (78 KB)Indeblues~commonswiki (talk | contribs){{Information |Description={{en|1=Chromatogram showing the UV absorption profile of Arabidopsis supernatant separated on Sephadex G-50 Superfine. Gel volume: 500 mL; column length: 700 mm; column diameter: 30 mm; eluent flow rate: 12 mL / hr; fraction vol

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