File:Differential-pH-dependent-cellular-uptake-pathways-among-foamy-viruses-elucidated-using-dual-1742-4690-9-71-S5.ogv

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Original file ‎(Ogg Theora video file, length 14 s, 786 × 740 pixels, 3.35 Mbps, file size: 5.49 MB)

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English: Movie. Uptake and trafficking of fusion-deficient PFV particles in host cells. HeLa cells were incubated with double-tagged PFV Gag-eGFP (non-spiked) and PE Ch iCS containing viral particles at low temperature (~10°C). After warming the cell to 37°C, z-stacks with 12 planes were recorded every 15 minutes for a total of 24 h using wide-field live-cell microscopy. The video shows the DIC channel (upper left), the red Ch-Env channel (upper right) and the green Gag-eGFP channel (lower left), both shown in gray, and a colored overlay of both channels with Gag-eGFP in green and Ch-Env in red (“merge”, lower right). The individual fluorescence channels and the “merge” channel are displayed as maximum intensity z-projection of each z-stack.
Date
Source Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D (2012). "Differential pH-dependent cellular uptake pathways among foamy viruses elucidated using dual-colored fluorescent particles". Retrovirology. DOI:10.1186/1742-4690-9-71. PMID 22935135. PMC: 3495412.
Author Stirnnagel K, Schupp D, Dupont A, Kudryavtsev V, Reh J, Müllers E, Lamb D, Lindemann D
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Date/TimeThumbnailDimensionsUserComment
current09:31, 20 November 201214 s, 786 × 740 (5.49 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 1.72 Mbps Completed 06:44, 24 August 2018 22 s
Streaming 720p (VP9) 1.72 Mbps Completed 21:56, 13 March 2024 1.0 s
VP9 480P 912 kbps Completed 06:44, 24 August 2018 14 s
Streaming 480p (VP9) 913 kbps Completed 13:23, 2 February 2024 2.0 s
VP9 360P 530 kbps Completed 06:44, 24 August 2018 13 s
Streaming 360p (VP9) 531 kbps Completed 19:12, 6 February 2024 1.0 s
VP9 240P 256 kbps Completed 06:44, 24 August 2018 7.0 s
Streaming 240p (VP9) 257 kbps Completed 21:24, 16 December 2023 1.0 s
WebM 360P 513 kbps Completed 15:49, 20 November 2012 8.0 s
Streaming 144p (MJPEG) 341 kbps Completed 08:27, 3 November 2023 8.0 s

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