File:Fast-Multiphase-Volume-Adaptation-to-Hyperosmotic-Shock-by-Escherichia-coli-pone.0035205.s003.ogv

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Fast-Multiphase-Volume-Adaptation-to-Hyperosmotic-Shock-by-Escherichia-coli-pone.0035205.s003.ogv ‎(Ogg Theora video file, length 2 min 15 s, 40 × 68 pixels, 3 kbps, file size: 47 KB)

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English: A sequence of raw fluorescent images of a single E.coli cell prior and during a hyperosmotic shock showing fast water efflux. The cell is transferred from isoosmotic buffer (10 mM Tris-HCl buffer supplemented with 150 mM sucrose) to isoosmotic buffer and 620 mM sucrose. Cytoplasmic volume was marked with the green protein EGFP and total cell volume by the red outer membrane dye FM4-64. Images were acquired at a frame every 1.6 seconds alternating between the cytoplasmic volume and total cell volume.
Date
Source Video S1 from Pilizota T, Shaevitz J (2012). "Fast, Multiphase Volume Adaptation to Hyperosmotic Shock by Escherichia coli". PLOS ONE. DOI:10.1371/journal.pone.0035205. PMID 22514721. PMC: 3325977.
Author Pilizota T, Shaevitz J
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current21:17, 30 October 20122 min 15 s, 40 × 68 (47 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 1 kbps Completed 07:52, 21 December 2023 1.0 s
WebM 360P 1 kbps Completed 09:38, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 19 kbps Completed 07:25, 11 November 2023 1.0 s

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