File:Imaging-plant-cell-death-GFP-Nit1-aggregation-marks-an-early-step-of-wound-and-herbicide-induced-1471-2229-5-4-S2.ogv

English: Additional File 2 Collapse of cells within the wound zone – wound induced granulation of GFP-Nit1 followed by cellular contraction and detachment from neighboring cells The cotyledons of agarose imbedded GFP-Nit1 lines (N1P2E) were wounded with razor incisions and subsequently imaged by collecting confocal Z-series of a region abutting the wound at 60 second intervals. (2A) Shown is a detail of a time-series made from brightest point 3-dimensional reconstructions of the acquired Z-series. Immediately after wounding, the center cell shown in the image series displayed GFP-Nit1 granules. After a short period of time, the cell started contracting with a extensive detachment of the cell from its cell walls. (2B) The full frame image data that Movie 2A was derived from. Note the numerous cells in the wound zone displaying granulation after wounding and their subsequent contraction over the course of the time series. Figure 2B in the manuscript was derived from this data set (see additional file 8).