File:Live-Imaging-at-the-Onset-of-Cortical-Neurogenesis-Reveals-Differential-Appearance-of-the-Neuronal-pone.0002388.s008.ogv
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DescriptionLive-Imaging-at-the-Onset-of-Cortical-Neurogenesis-Reveals-Differential-Appearance-of-the-Neuronal-pone.0002388.s008.ogv |
English: Two-photon laser scanning time-lapse video microscopy of acute 500 µm-thick slice culture prepared from dorsal telencephalon of E12.5 Tis21-nucGFP/Tubb3-mGFP double transgenic mouse embryos; time-lapse interval 12 min; maximum intensity projection of the single focal planes (2.7-µm steps) that showed the cells of interest. A Tis21-nucGFP-positive neurogenic progenitor migrates rapidly from the basal boundary of the ventricular zone to the apical surface and divides. The nuclei of the daughter cells migrate towards the basal side, with one nucleus leading . The leading nucleus was tracked until it entered the neuronal layer, whereas the trailing nucleus was tracked until it disappeared due to photobleaching.
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Source | Movie S3 from Attardo A, Calegari F, Haubensak W, Wilsch-Bräuninger M, Huttner W, Reh T. "Live Imaging at the Onset of Cortical Neurogenesis Reveals Differential Appearance of the Neuronal Phenotype in Apical versus Basal Progenitor Progeny". PLOS ONE. DOI:10.1371/journal.pone.0002388. PMID 18545663. PMC: 2398773. | ||
Author | Attardo A, Calegari F, Haubensak W, Wilsch-Bräuninger M, Huttner W, Reh T | ||
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:14, 31 August 2012 | 23 s, 303 × 711 (747 KB) | Open Access Media Importer Bot (talk | contribs) | Uploaded with the Open Access Media Importer. (test edit) botrequest |
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Short title | Movie S3 |
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Author | |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-06-11 |