File:Monitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s009.ogv
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Monitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s009.ogv (Ogg Theora video file, length 16 s, 400 × 568 pixels, 4.55 Mbps, file size: 8.44 MB)
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DescriptionMonitoring-the-Cytoskeletal-EGF-Response-in-Live-Gastric-Carcinoma-Cells-pone.0045280.s009.ogv |
English: Paxillin is recruited to focal adhesions prior to tyrosine phosphorylation in newly-formed lamella upon EGF treatment (see also Fig. 7). The images are taken from a MKN1 cell simultaneously expressing paxillin-dsRed and CFP-dSH2 (false green color) immediately after addition of 5 ng/ml EGF. The composite Video presents the overlays of paxillin-dsRed and CFP-dSH2 (top) and the overlays in combination with DIC micrographs (bottom). Images were recorded every 25 s by confocal laser scanning microscopy. Note the appearance of paxillin-positive focal adhesion sites in the proceding lamellum prior to dSH2 recruitment. |
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Source | Video S9 from Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R (2012). "Monitoring the Cytoskeletal EGF Response in Live Gastric Carcinoma Cells". PLOS ONE. DOI:10.1371/journal.pone.0045280. PMID 23028903. PMC: 3459943. | ||
Author | Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 16:41, 30 October 2012 | 16 s, 400 × 568 (8.44 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Felkl M, Tomas K, Smid M, Mattes J, Windoffer R, Leube R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Paxillin is recruited to focal adhesions prior to tyrosine phosphorylation in newly-formed lamella upon EGF treatment (see also Fig. 7). The images are taken from a MKN1 cell simultaneously expressing paxillin-dsRed and CFP-dSH2 (false green color) immediately after addition of 5 ng/ml EGF. The composite Video presents the overlays of paxillin-dsRed and CFP-dSH2 (top) and the overlays in combination with DIC micrographs (bottom). Images were recorded every 25 s by confocal laser scanning microscopy. Note the appearance of paxillin-positive focal adhesion sites in the proceding lamellum prior to dSH2 recruitment. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |