File:MultiPhotonExcitation-Fig10-doi10.1186slash1475-925X-5-36.JPEG

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English: Original figure legend: Multiple fluorescence 2PE imaging. 2PE multiple fluorescence image from a 16 μm cryostat section of mouse intestine stained with a combination of fluorescent stains (F-24631, Molecular Probes). Alexa Fluor 350 wheat germ agglutinin, a blue-fluorescent lectin, was used to stain the mucus of goblet cells. The filamentous actin prevalent in the brush border was stained with red-fluorescent Alexa Flu or 568 phalloidin. Finally, the nuclei were stained with SYTOX ® Green nucleic acid stain. Imaging has been performed at 780 nm, 100 x 1.4 NA Leica objective, using a Chameleon XR ultrafast Ti-Sapphire laser (Coherent Inc., USA) coupled at LAMBS-MicroScoBio with a Spectral Confocal Laser Scanning Microscope, Leica SP2-AOBS.
Deutsch: Zweiphotonenaufnahme an einem Schnitt durch einen Mausdarm. Zellkerne in grün, Schleim der Becherzellen in blau, Aktin (Phalloidin-Färbung) in rot. Anregung erfolgte bei 780 nm durch einen Titan:Saphir-Laser.
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Multi-photon excitation microscopy. BioMedical Engineering OnLine, 2006, 5:36.
DOI:10.1186/1475-925X-5-36.

http://www.biomedical-engineering-online.com/content/5/1/36
Author Alberto Diaspro, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino and Cesare Usai
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