File:PCR.svg

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PCR.svg(SVG file, nominally 800 × 1,800 pixels, file size: 111 KB)

Description
Nederlands: Nederlands: Schematische voorstelling van de PCR ketingreactie.
  1. De denaturatie van de de dubbele helix naar een enkele DNA-streng bij 96 °C.
  2. hybridizatie van de enkelstreng met primers bij 68 °C.
  3. vervollediging van de DNA-enkelstrengen bij 72 °C (p= polymerase).
  4. Eind van de eerste cyclus.
in de praktijk wordt deze cyclus vele malen herhaald.


English: Diagram of PCR reaction to demonstrate how amplification leads to the exponential growth of a short product flanked by the primers.


Schematic drawing of the PCR cycle.

  1. Denaturing at 96°C.
  2. Annealing at 68°C.
  3. Elongation at 72°C (P=Polymerase).
  4. The first cycle is complete. The two resulting DNA strands make up the template DNA for the next cycle, thus doubling the amount of DNA duplicated for each new cycle.


Deutsch: Schematische Darstellung des PCR-Zyklus.
  1. Denaturalisieren/Schmelzen bei 96 °C.
  2. Anlagerung bei 68 °C.
  3. Verlängerung bei 72 °C (P=Polymerase).
  4. Der erste Zyklus ist beendet.
Date

22 March 2007(2007-03-22)

Source

Own work

Author

Madprime

Permission
(Reusing this image)

GFDL and CC-BY-SA

[edit] Licensing

I, the copyright holder of this work, hereby publish it under the following licenses:
GNU head Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation License".

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This file is licensed under the Creative Commons Attribution ShareAlike 3.0 License. In short: you are free to share and make derivative works of the file under the conditions that you appropriately attribute it, and that you distribute it only under a license identical to this one. Official license

This licensing tag was added to this file as part of the GFDL licensing update.


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Creative Commons license
Creative Commons Attribution Creative Commons Share Alike
This file is licensed under the Creative Commons Attribution ShareAlike 2.5, Attribution ShareAlike 2.0 and Attribution ShareAlike 1.0 License. In short: you are free to share and make derivative works of the file under the conditions that you appropriately attribute it, and that you distribute it only under a license identical to this one.

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File history

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Date/TimeThumbnailDimensionsUserComment
current13:35, 2 May 2008Thumbnail for version as of 13:35, 2 May 2008800×1,800 (111 KB)Madprime (Talk | contribs) ({{Information| |Description={{nl|1= Nederlands: Schematische voorstelling van de PCR ketingreactie. # De denaturatie van de de dubbele helix naar een enkele DNA-streng bij 96 °C. # hybridizatie van de enkelstreng met primers bij 68 °C. # vervollediging )
16:15, 28 March 2007Thumbnail for version as of 16:15, 28 March 2007800×1,800 (111 KB)Madprime (Talk | contribs) (flipped shading on polymerase)
00:01, 23 March 2007Thumbnail for version as of 00:01, 23 March 2007800×1,800 (111 KB)Madprime (Talk | contribs) (Diagram of PCR reaction to demonstrate how amplification leads to the exponential growth of a short product flanked by the primers.)
23:52, 22 March 2007Thumbnail for version as of 23:52, 22 March 2007800×1,800 (98 KB)Madprime (Talk | contribs) (Diagram of PCR reaction to demonstrate how amplification leads to the exponential growth of a short product flanked by the primers.)

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