File:RNA-in-situ-dark-field.jpg

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RNA-in-situ-dark-field.jpg ‎(364 × 198 pixels, file size: 62 KB, MIME type: image/jpeg)

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English: Tissue section with radioactive RNA in situ hybridization followed by coating with autoradiographic emulsion, development of silver grains and hematoxylin staining. See Methods section of original paper for details.

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Localization of expression of mRNA encoding TGF-βs in ovine ovaries. Panels a and b contain corresponding light field and dark field views of a type 5 follicle from an adult ewe hybridized to TGF-β1 antisense RNA. Silver grains indicating hybridization of the TGF-β1 antisense RNA are observed concentrated in thecal (t) cells close to the basement membrane with no specific hybridization observed in either the granulosa cells (gc) or oocyte (o). Scale bar equals approximately 100 μm.
Deutsch: Gewebeschnitt nach radioaktiver RNA in situ Hybridisierung und Nachweis der Radioaktivität durch Entstehung von Silberkörnern. Bei Hellfeld-Mikroskopie (links) sind diese kaum zu sehen, bei Dunkelfeldmikroskopie (rechts) treten sie dagegen deutlich hervor.
Date
Source Reproductive Biology and Endocrinology 2004, 2:78 doi:10.1186/1477-7827-2-78. Figure 1. Online
Author Jennifer L Juengel, Adrian H Bibby, Karen L Reader, Stan Lun, Laurel D Quirke, Lisa J Haydon and Kenneth P McNatty
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