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A-negative-feedback-loop-controls-NMDA-receptor-function-in-cortical-interneurons-via-neuregulin-ncomms8222-s3.ogv (Ogg Theora video file, length 25 s, 512 × 512 pixels, 903 kbps)
English: Supplementary Movie 2 Time-lapse of the NMDAR-dependent downregulation of Venus-NRG2 clusters in response to glutamate. Neurons were infected at DIV3 with Venus-NRG2 and live-imaged on DIV 21 (see Methods). The movie depicts representative neurons shown in Figure 3, with individual frames acquired every 30 seconds. Baseline fluorescence was acquired for 5 min, either in the absence (S2) or presence (S3) of 50 μM AP5, followed by bath application of 20 μM glutamate for 10 min. In movie S2, Venus-NRG2 clusters dim immediately after the addition of glutamate and quickly fade to background levels. In movie S3, Venus-NRG2 fluorescence remains stable throughout the duration of the recording session due to the blockade of NMDA receptors by AP5.
|Source||Video file from Vullhorst D, Mitchell R, Keating C, Roychowdhury S, Karavanova I, Tao-Cheng J, Buonanno A (2015). "A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling". Nature Communications. DOI:10.1038/ncomms8222. PMID 26027736. PMC: 4451617.|
|Author||Vullhorst D, Mitchell R, Keating C, Roychowdhury S, Karavanova I, Tao-Cheng J, Buonanno A|
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|current||03:35, 1 November 2016||25 s, 512 × 512 (2.72 MB)||Open Access Media Importer Bot||Automatically uploaded media file from Open Access source. Please report problems or suggestions here.|
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