File:A-negative-feedback-loop-controls-NMDA-receptor-function-in-cortical-interneurons-via-neuregulin-ncomms8222-s3.ogv

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Summary[edit]

Description
English: Supplementary Movie 2 Time-lapse of the NMDAR-dependent downregulation of Venus-NRG2 clusters in response to glutamate. Neurons were infected at DIV3 with Venus-NRG2 and live-imaged on DIV 21 (see Methods). The movie depicts representative neurons shown in Figure 3, with individual frames acquired every 30 seconds. Baseline fluorescence was acquired for 5 min, either in the absence (S2) or presence (S3) of 50 μM AP5, followed by bath application of 20 μM glutamate for 10 min. In movie S2, Venus-NRG2 clusters dim immediately after the addition of glutamate and quickly fade to background levels. In movie S3, Venus-NRG2 fluorescence remains stable throughout the duration of the recording session due to the blockade of NMDA receptors by AP5.
Date
Source Video file from Vullhorst D, Mitchell R, Keating C, Roychowdhury S, Karavanova I, Tao-Cheng J, Buonanno A (2015). "A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling". Nature Communications. DOI:10.1038/ncomms8222. PMID 26027736. PMC: 4451617.
Author Vullhorst D, Mitchell R, Keating C, Roychowdhury S, Karavanova I, Tao-Cheng J, Buonanno A
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