File:Automated-Screening-of-Microtubule-Growth-Dynamics-Identifies-MARK2-as-a-Regulator-of-Leading-Edge-pone.0041413.s011.ogv

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English: EB3 dynamics in U2-OS cells treated with shRNAs targeting MARK2. Spinning disk confocal fluorescence time-lapse image sequences of cells expressing mKO-EB3 to mark growing MT plus ends in cells expressing shRNA targeting MARK2 (left), CA-Rac1 and shRNA targeting MARK2 (middle) or expressing CA-Rac1, shRNA targeting MARK2, and RNAi-resistant GFP-MARK2 (right). The same cells are shown in Figure 4F and Figure 5E. Bar  = 10 µm, elapsed time in min: sec.
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Source Video S2 from Nishimura Y, Applegate K, Davidson M, Danuser G, Waterman C (2012). "Automated Screening of Microtubule Growth Dynamics Identifies MARK2 as a Regulator of Leading Edge Microtubules Downstream of Rac1 in Migrating Cells". PLOS ONE. DOI:10.1371/journal.pone.0041413. PMID 22848487. PMC: 3404095.
Author Nishimura Y, Applegate K, Davidson M, Danuser G, Waterman C
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