File:Early-Signaling-in-Primary-T-Cells-Activated-by-Antigen-Presenting-Cells-Is-Associated-with-a-Deep-pone.0133299.s004.ogv

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Early-Signaling-in-Primary-T-Cells-Activated-by-Antigen-Presenting-Cells-Is-Associated-with-a-Deep-pone.0133299.s004.ogv ‎(Ogg Theora video file, length 14 s, 83 × 107 pixels, 52 kbps, file size: 89 KB)

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English: Representative interactions of 5C.C7 T cells retrovirally transduced to express the indicated sensors with CH27 B cell lymphoma APCs in the presence of MCC agonist peptide (10 M) are shown in S1 to S3 Videos. DIC images are shown on the top, with matching top-down, maximum projections of 3D sensor fluorescence data on the bottom. The sensor fluorescence intensity is displayed in a rainbow-like, false-color scale (increasing from blue to red). 20 s intervals in video acquisition are played back as 2 frames per second. The 5C.C7 T cell in S1 Video is transduced with SLP-76-GFP. Cell coupling occurs in frame 5 (2s indicated video time). A rapid transition from central to lamellal accumulation that subsequently fades more slowly is shown.
Date
Source S1 Video from Roybal K, Mace E, Mantell J, Verkade P, Orange J, Wülfing C (2015). "Early Signaling in Primary T Cells Activated by Antigen Presenting Cells Is Associated with a Deep and Transient Lamellal Actin Network". PLOS ONE. DOI:10.1371/journal.pone.0133299. PMID 26237050. PMC: 4523204.
Author Roybal K, Mace E, Mantell J, Verkade P, Orange J, Wülfing C
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current01:12, 11 August 201514 s, 83 × 107 (89 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 9 kbps Completed 14:01, 17 December 2023 1.0 s
WebM 360P 7 kbps Completed 06:53, 30 November 2023 0.0 s
Streaming 144p (MJPEG) 44 kbps Completed 14:45, 9 November 2023 0.0 s

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