File:Externalized-decondensed-neutrophil-chromatin-occludes-pancreatic-ducts-and-drivespancreatitis-ncomms10973-s6.ogv

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Original file ‎(Ogg multiplexed audio/video file, Theora/Vorbis, length 7.6 s, 1,920 × 1,080 pixels, 3.88 Mbps overall, file size: 3.53 MB)

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English: Supplementary Movie 5 Video microscopy of human neutrophils from healthy donors. Neutrophils were

allowed to adhere to the surface of an IbidiTreat-coated Ibidi Flow Chamber (Ibidi, Munich, Germany) for 30 minutes at 37°C / 5% CO2 before supplementation with 75 mM NaHCO3 and further incubation for 120 minutes. DNA was detected by Sytox Green. The total time lapse of the movie is 2 minutes compressed to 7 seconds (16x). Please note the tangles of extracellular DNA derived from granulocytes with non-nuclear decondensed morphology, which are attached to the surface and slightly move in the flow. Further note, that threads of free DNA are captured by immobilized DNA aggregates, increasing the size of the latter (n=3 independent experiments;

Movie 5 provided as online movie file only).
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Source Video file from Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C (2016). "Externalized decondensed neutrophil chromatin occludes pancreatic ducts and drives pancreatitis". Nature Communications. DOI:10.1038/ncomms10973. PMID 26964500. PMC: 4793047.
Author Leppkes M, Maueröder C, Hirth S, Nowecki S, Günther C, Billmeier U, Paulus S, Biermann M, Munoz L, Hoffmann M, Wildner D, Croxford A, Waisman A, Mowen K, Jenne D, Krenn V, Mayerle J, Lerch M, Schett G, Wirtz S, Neurath M, Herrmann M, Becker C
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