File:ICP0-Dismantles-Microtubule-Networks-in-Herpes-Simplex-Virus-Infected-Cells-pone.0010975.s007.ogv

English: ICP0ΔRING accumulates in linear cytoplasmic structures in HSV-1 0ΔRING-infected cells. Time-lapse photographs of ICP0ΔRING protein in a representative field of Vero cells between 4.0 and 4.5 hours post-release from a cycloheximide block. Vero cells were inoculated with 5 pfu per cell of HSV-1 0ΔRING in the presence of 200 µM cycloheximide from −0.5 to 10 hours p.i., and were released into medium containing no drugs. At 4.0 hours post-release, a field of cells was chosen where ICP0ΔRING was present in linear cytoplasmic structures. Cultures were continuously incubated at 37°C before and during time-lapse photography. Photographs were captured at a rate of twice per minute for 30 minutes, and are shown in this video at an elapsed rate that is 150 times faster than normal.