File:Mapping-molecules-in-scanning-far-field-fluorescence-nanoscopy-ncomms8977-s2.ogv

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Original file(Ogg Theora video file, length 36 s, 1,600 × 840 pixels, 1.96 Mbps)

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Description
English: Supplementary Movie 1 A 3D molecular map of the TfR (stained with aptamer in HEK293 cells) is reconstructed from the photon statistics of confocal and STED measurements. Isosurfaces of the molecular map which embrace 70% of the overall number of molecules in the corresponding cluster are plotted in the animation. The cluster segmentation is performed with the built in watershed function in MATLAB. 0 - 5 s: reconstruction from the photon statistics of confocal recording (Red). 6 - 11 s: reconstruction from the photon statistics of confocal and STED recording (Cyan). 19 - 31 s: reconstruction from the photon statistics of confocal and STED recording with color code representing the number of molecules in each cluster. The full region is 8 x 8 x 2 μm.
Date
Source Video file from Ta H, Keller J, Haltmeier M, Saka S, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell S (2015). "Mapping molecules in scanning far-field fluorescence nanoscopy". Nature Communications. DOI:10.1038/ncomms8977. PMID 26269133. PMC: 4557268.
Author Ta H, Keller J, Haltmeier M, Saka S, Schmied J, Opazo F, Tinnefeld P, Munk A, Hell S
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current09:31, 31 October 201636 s, 1,600 × 840 (8.33 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.
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