File:Microplastics that have penetrated inside cells.png
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DescriptionMicroplastics that have penetrated inside cells.png |
English: Microplastics measuring 0.5 μm penetrate into the cytoplasm of MH-22a hepatocyte cells. The cytoskeleton is labeled with green fluorescent phalloidin, the nucleus is stained with blue fluorescent DAPI, and cell fixation is achieved using orange formaldehyde.
This intriguing observation suggests the possibility of microplastic penetration into cells and its impact on cellular processes. However, further investigations are necessary to gain a more precise understanding of the effects and mechanisms associated with microplastic internalization into cells and its influence on the cytoskeleton and cellular structures. Your observation visualizes the presence of microplastics within cells and their interaction with the cytoskeleton. Phalloidin, used for cytoskeletal staining, enables visualization of actin filaments, a major component of the cytoskeleton, in green fluorescence. DAPI staining of the nucleus in blue fluorescence aids in nucleus localization, while orange formaldehyde is employed for cell fixation and preservation of cellular structure. Such observations hold significance for future research and contribute to a better understanding of the impact of microplastics on cells and organisms. This field represents an area of active investigation where additional studies are being conducted to comprehensively comprehend the implications of microplastic presence within cells and its potential effects on health and the environment.Русский: Микропластик размером 0,5 мкм проникает внутрь цитоплазмы гепатоцитов линии MH-22а. Цитоскелет окрашен зеленым флуорофором фаллоидином, ядро окрашено синим флуорофором DAPI, а фиксация клеток выполнена с использованием оранжевого формальдегида.
Это интересное наблюдение, которое может указывать на возможность проникновения микропластика внутрь клеток и его влияние на клеточные процессы. Однако, для более точного понимания эффектов и механизмов, связанных с проникновением микропластика в клетки и его воздействием на цитоскелет и клеточные структуры, требуется проведение дополнительных исследований. Ваше наблюдение визуализирует присутствие микропластика внутри клетки и его взаимодействие с цитоскелетом. Фаллоидин, используемый для окрашивания цитоскелета, позволяет визуализировать актиновые филаменты, одну из основных компонент цитоскелета, в зеленом цвете. Окрашивание ядра DAPI синим флуорофором позволяет локализовать ядра клеток, а оранжевый формальдегид используется для фиксации клеток и сохранения их структуры. Наблюдения такого рода имеют значение для дальнейших исследований и позволяют лучше понять влияние микропластика на клетки и организмы. Это одна из областей активных исследований, где проводятся дополнительные работы для более полного понимания последствий присутствия микропластика внутри клеток и его потенциального влияния на здоровье и окружающую среду.English: The image shows microplastics with a diameter of 0.5 μm (small green spheres) penetrating the cytoplasm of MH-22a hepatocyte cells. ZEISS Axio Imager 2 |
Date | |
Source | Own work |
Author | Karimov Denis, Valova Iana |
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This image was uploaded as part of Russian Science Photo Competition 2023.
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current | 07:20, 26 May 2023 | 2,584 × 1,936 (3.04 MB) | Karimovdo (talk | contribs) | Uploaded own work with UploadWizard |
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