File:Molecular-Basis-for-the-Dual-Function-of-Eps8-on-Actin-DynamicsBundling-and-Capping-pbio.1000387.s020.ogv
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Molecular-Basis-for-the-Dual-Function-of-Eps8-on-Actin-DynamicsBundling-and-Capping-pbio.1000387.s020.ogv (Ogg Theora video file, length 8.8 s, 500 × 496 pixels, 290 kbps, file size: 312 KB)
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DescriptionMolecular-Basis-for-the-Dual-Function-of-Eps8-on-Actin-DynamicsBundling-and-Capping-pbio.1000387.s020.ogv |
English: Propulsion rate of rocketing PIP2-rich vesicles in the presence of EPS8-Δbund. Time-lapse fluorescent microscopy of PIP2-rich vesicles was performed as described in Figure S5 by expressing PI4,5K together with EPS8-Δbund. Only the dynamic of lifeact-cherry is shown to facilitate the visualization of rocketing vesicles. Frames were taken every 3.3 to 7.1 s for a total time period of 5 min using a Spinning Disk confocal microscope Ultra VIEW VoX, Perkin Elmer 100× objective, 1.49 N.A, Nikon TiEclipse. |
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Source | Video S10 from Hertzog M, Milanesi F, Hazelwood L, Disanza A, Liu H, Perlade E, Malabarba M, Pasqualato S, Maiolica A, Confalonieri S, Le Clainche C, Offenhauser N, Block J, Rottner K, Di Fiore P, Carlier M, Volkmann N, Hanein D, Scita G (2010). "Molecular Basis for the Dual Function of Eps8 on Actin Dynamics: Bundling and Capping". PLOS Biology. DOI:10.1371/journal.pbio.1000387. PMID 20532239. PMC: 2879411. | ||
Author | Hertzog M, Milanesi F, Hazelwood L, Disanza A, Liu H, Perlade E, Malabarba M, Pasqualato S, Maiolica A, Confalonieri S, Le Clainche C, Offenhauser N, Block J, Rottner K, Di Fiore P, Carlier M, Volkmann N, Hanein D, Scita G | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:10, 5 June 2013 | 8.8 s, 500 × 496 (312 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Hertzog M, Milanesi F, Hazelwood L, Disanza A, Liu H, Perlade E, Malabarba M, Pasqualato S, Maiolica A, Confalonieri S, Le Clainche C, Offenhauser N, Block J, Rottner K, Di Fiore P, Carlier M, Volkmann N, Hanein D, Scita G |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010-06 |