File:PDNA transfer via PepFect14.jpg

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Original file ‎(3,644 × 2,256 pixels, file size: 4.93 MB, MIME type: image/jpeg)

Captions

Add a one-line explanation of what this file represents

Captions

Summary[edit]

Description
Eesti: CHO rakku tuuma on viidud plasmiidset DNA-d (pGL3; 4,7 kDa) kasutades selleks PepFect14 rakku sisenevaid peptiide. Vasakul äärel on näha rakutuum, paremal mitokondrid. pDNA on märgendatud hõbetatud nanokullaga ja pildil osundab sellele nool. Transmissioonelektronmikroskoopia. Tartu Ülikooli molekulaar- ja rakubioloogia instituut. [1]

Plasmiidse DNA puhul moodustati esmalt pDNA ja streptavidiin-nanokuld (SA-NG) kompleksid, et pDNA oleks elektronmikroskoobis paremini detekteeritav. Selleks inkubeeriti eelnevalt biotinüleeritud pDNA molekule SA-NG konjugaatidega molekulaarsel suhtel 1:3 (pDNA:SANG) 30 min toatemperatuuril. Seejärel inkubeeriti pDNA-SA-NG komplekse PepFect14-ga laengusuhtel 1:2 30 min toatemperatuuril. Seejärel lisati kompleksidele 900 μl söödet, saavutades lõppruumala 1 ml. Vahetult enne komplekside rakkudele kandmist pesti neid kaks korda söötmega ning seejärel kanti rakkudele peale inkubatsioonilahus. Rakke inkubeeriti 37°C juures 4 h. Rakud fikseeriti glutaaraldehüüdiga 0,2 M Na-kakodülaatpuhvris (pH 7,4). Suurendamaks kullamärgist (1,4 nm) hõbetati rakke saavutades nõnda märgiste läbimõõt ~10–15 nm. Rakud on kontrasteeritud osmiumtetroksiidiga.
English: Transmission electron micrograph of a CHO cell, that has plasmid DNA transferred into its nucleus with PepFect14 cell-penetrating peptides. On the right side of the image there are mitochondria. pDNA (pGL3; 4,7 kDa) has been marked with nanogold, that has been enlarged with silver coating to make it visible under the microscope (coated nanogold marker is indicated with an arrow). Institute of Molecular and Cell Biology, University of Tartu.
Date
Source Own work
Author Ivo Kruusamägi

Licensing[edit]

I, the copyright holder of this work, hereby publish it under the following license:
w:en:Creative Commons

attribution share alike

This file is licensed under the Creative Commons Attribution-Share Alike 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current22:34, 25 November 2016Thumbnail for version as of 22:34, 25 November 20163,644 × 2,256 (4.93 MB)Kruusamägi (talk | contribs)User created page with UploadWizard

You cannot overwrite this file.

The following page uses this file:

File usage on other wikis

The following other wikis use this file:

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:PDNA_transfer_via_PepFect14.jpg&oldid=441665048"