File:Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s005.ogv

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Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s005.ogv(Ogg Theora video file, length 6.2 s, 800 × 600 pixels, 1.02 Mbps)

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Description
English: Live cell video of cell motility of Plxnb2−/− macrophages. Representative cell movement video over a 2.5 hour time period. 1×104 cells were plated on glass bottom dishes (MatTek Ashland, MA P35G-1.5-10-C) in complete DMEM, rested overnight, and treated with sham (warmed DMEM). Cells were imaged on a Nikon Biostation (Belmont, CA) with a 20× objective at ten different points. Images were collected every 5 minutes for a total time of 2.5 hours following sham treatment. Three separate movies were filmed and 45 cells scored for velocity. Cell velocity was analyzed using manual tracking in ImageJ [76]. The experiment was repeated three separate times using cells from different mice in each experiment.
Date
Source Video S3 from Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J (2011). "Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation". PLOS ONE. DOI:10.1371/journal.pone.0024795. PMID 21966369. PMC: 3179467.
Author Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J
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current03:29, 14 November 20126.2 s, 800 × 600 (775 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.
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