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Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s005.ogv (Ogg Theora video file, length 6.2 s, 800 × 600 pixels, 1.02 Mbps)
English: Live cell video of cell motility of Plxnb2−/− macrophages. Representative cell movement video over a 2.5 hour time period. 1×104 cells were plated on glass bottom dishes (MatTek Ashland, MA P35G-1.5-10-C) in complete DMEM, rested overnight, and treated with sham (warmed DMEM). Cells were imaged on a Nikon Biostation (Belmont, CA) with a 20× objective at ten different points. Images were collected every 5 minutes for a total time of 2.5 hours following sham treatment. Three separate movies were filmed and 45 cells scored for velocity. Cell velocity was analyzed using manual tracking in ImageJ . The experiment was repeated three separate times using cells from different mice in each experiment.
|Source||Video S3 from Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J (2011). "Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation". PLOS ONE. DOI:10.1371/journal.pone.0024795. PMID 21966369. PMC: 3179467.|
|Author||Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J|
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|current||03:29, 14 November 2012||6.2 s, 800 × 600 (775 KB)||Open Access Media Importer Bot||Automatically uploaded media file from Open Access source. Please report problems or suggestions here.|
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