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English: RLCA20A21-GFP is not localized to the equatorial cortex and cells do not contract. Drosophila S2 cells expressing RLCA20A21-GFP (green) and mRFP-alpha-tubulin (red) were treated with dsRNA for 5 days to deplete the endogenous RLC. Epifluorescence microscopy was used to film cells as they transitioned from metaphase into anaphase and telophase. Images were taken at 20 second intervals and movies are played at 10 frames/second. Figure 2C, row 2 contains images from this movie.
Source Movie S2 from Dean S, Spudich J (2006). "Rho Kinase's Role in Myosin Recruitment to the Equatorial Cortex of Mitotic Drosophila S2 Cells Is for Myosin Regulatory Light Chain Phosphorylation". PLOS ONE. DOI:10.1371/journal.pone.0000131. PMID 17205135. PMC: 1762308.
Author Dean S, Spudich J
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