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English: Flow defects in GRP explants from Wnt11b manipulated embryos. Movie shows time-lapse sequences of dorsal explants to which fluorescent beads were added (cf. Figure 2A-C). Specimens were mounted dorsal side down and viewed from the ventral side, anterior to the top. Movie represents a total length of 500 frames taken at a rate of 2 frames/sec and runs at 40×real time. Opening frame displays bright field images and indicates orientation of GRP (dashed lines). Videos were processed to yield gradient time trails (GTTs), i.e. color-coded tracks of beads which revealed direction of transport and velocity of particles (from green to red; 25 s). Note that robust leftward flow (uninjected controls) was impaired in Wnt11b morphant and upon injection of wild-type Wnt11b DNA.
Source Movie S1 from Walentek P, Schneider I, Schweickert A, Blum M (2013). "Wnt11b Is Involved in Cilia-Mediated Symmetry Breakage during Xenopus Left-Right Development". PLOS ONE. DOI:10.1371/journal.pone.0073646. PMID 24058481. PMC: 3772795.
Author Walentek P, Schneider I, Schweickert A, Blum M
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