User talk:Mariuswalter

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Welcome to Wikimedia Commons, Mariuswalter!

-- Wikimedia Commons Welcome (talk) 13:59, 1 July 2015 (UTC)

Image formats[edit]

Thanks for providing some nice illustrations related to histones! You created them as PDF format, which has some portability and security problems. Wikipedia recommends SVG (if you have vector content, potentially translatable text, etc) or PNG (if you have pure graphics). I don't know what programs you used to create the images, so I don't know whether it's simply a matter of choosing a different format when saving, or would require some other conversion. Or whether there actually is a technical reason why PDF is a better choice for these (there are some cases I think). Let me know if you need more information. DMacks (talk) 21:37, 1 July 2015 (UTC)

Hi Dmacks, I'd be happy to provide the same illustration in svg. I just join yesterday and I didn't knew there was a problem with pdf. I tried however to upload the svg version (with the "upload a new version of this file" link) but it didn't work. I think the problem is that the file name has a "pdf" extension and that it generate a conflict. And it looks like I cannot modify the filename myself or delete the file. could you help me doing that ? Or tell me how to do it ? And I'm probably going to have the issue with the other .pdf file I uploaded yesterday.
Thank for you help ! Mariuswalter (talk) 11:50, 2 July 2015 (UTC)
The extension is actually part of the filename for purposes of wikimedia software. So "FOO.svg" and "FOO.pdf" are totally independent entities, not multiple revisions of each other at a common name called "FOO". Simply upload the svg as a brand new file with that name rather than as a new version of the pdf file. After the new file exists, you (or I or someone else:) can change the links in the wikipedia articles to use the new filenames. Once the old files are no longer in-use, there's a way to mark them for eventual deletion. DMacks (talk) 18:42, 4 July 2015 (UTC)

Possible image inconsistency?[edit]

The images you have done for the CRISPR page [1] are really nice! However I think I have found an inconsistency. The top figure [2] is a little unconventional in that the "top" strand of DNA is 3' -> 5'. I guess you have done this because it allows you to represent the gRNA 5'->3', making it easier to illustrate the PAM sequence. I think it generates an inconsistency with the top section of the figure further down the page [3]: here it appears that the wrong strand of DNA is acting as template for RNA synthesis given the direction in which the RNA polymerase is shown to be moving/the transcript is emerging. Given the strength of the convention for having the top strand of DNA be 5'->3' it might be worth considering making the harder edit of [2] rather than the simpler edit of [3] to remove the inconsistency. I'm looking forward to using your images for teaching (or course with attribution). Many thanks Chanoyu (talk) 02:49, 24 October 2017 (UTC)

Thanks for your message! Indeed there is an inconsistency, thanks for pointing that out. I will try to find a way to fix it as soon as I can. Knowing that my images are useful to people is more than enough for me, you can simply cite wikipedia :) Mariuswalter (talk) 00:17, 25 October 2017 (UTC)
Hi again @Chanoyu: So in the end I went for the easier edit and removed the inconsistency on the second figure. It makes transcription highly unusual since it basically goes reverse-strand with the 3'-strand on top, but at least it is biologically correct (hopefully!). I am aware that it strongly goes against the convention, but this is for the sake of clarity. I tried very hard to re-do the figure [2] with the top strand 5'->3', but I cannot find a way to make it as nice as the first one... Here my point was really to provide good figures of CRISPR-Cas and of some applications, and I don't want to make the figures more messy than they should be. I hope you see my point!
That being said, I just made other versions of the figures with 5'->3' on top, and even if I don`t want to put them online, I would be very happy to share them with you if you feel more confortable using them for teaching. I can also give you illustrator and pdf version of all the files, so you can modify them at will. Mariuswalter (talk) 20:20, 25 October 2017 (UTC)
Hi @Mariuswalter: Thanks for making the changes so quickly - I've updated my slides! It would be great to have the editable variations - are you at the Buck Inst? Many thanks Chanoyu (talk) 19:46, 26 October 2017 (UTC)
@Chanoyu:, I am! mwalter@buckinstitute.org Mariuswalter (talk) 20:12, 26 October 2017 (UTC)

[1] https://en.wikipedia.org/wiki/CRISPR [2] https://upload.wikimedia.org/wikipedia/commons/5/51/GRNA-Cas9.png [3] https://upload.wikimedia.org/wikipedia/commons/5/51/Dead-Cas9_potential_applications.png — Preceding unsigned comment added by Chanoyu (talk • contribs) 02:46, 24 October 2017 (UTC)