File:Journal.pone.0031641.g003.png
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DescriptionJournal.pone.0031641.g003.png |
English: LGs start being secreted before establishment of paracellular permeability barrier.
(A, B) TJ permeability assay using a surface biotinylation technique. LG molecules, CDSN and glucosylceramides (GlcCer) are co-localized with biotin (areas between arrowheads). A part of CDSN/biotin image in A is magnified in A′ and the points where the tracer stops are indicated with yellow arrows. The basement membrane zone is highlighted by the broken line. (C, D) Lanthanum penetration assay on normal human skin. (C) Lanthanum penetration is blocked at the TJ (bracket) in the SG1. (D) Secreted LGs (red arrows) can be found in the tracer permeable intercellular space. Green arrows indicate LGs in the cytoplasm. The area in the rectangle with broken lines in D is magnified in D′. D, desmosome. Bars = 10 µm (A, A′, B), 200 nm (C, D′) and 500 nm (D). |
Date | |
Source | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0031641 |
Author | Akemi Ishida-Yamamoto, Mari Kishibe, Masamoto Murakami, Masaru Honma, Hidetoshi Takahashi, Hajime Iizuka |
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