File:Limbal epithelial cells. Monolayer. SEM-BSE.jpg
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Русский: Клетки лимбального эпителия роговицы, сформировавшие монослой. Изображение в обратно-рассеянных электронах (BSE), полученное после контрастирования лантаноидами (BioREE, Россия) на сканирующем электронном микроскопе EVO LS10 (Zeiss, Германия) в режиме низкого вакуума без фиксации, обезвоживания и напыления образца (лаборатория фундаментальных исследований ФГБНУ «НИИ глазных болезней»). Видны ядра с ядрышками, компоненты внеклеточного вещества. Очень ярко светятся границы клеток — насыщенные при контрастировании редкоземельными элементами активные межклеточные контакты. При таком способе пробоподготовки и визуализации, структуры в подповерхностном слое клеток очень похожи на пузырьки воздуха в толще льда замерзшего пруда - чем объекты глубже, тем они менее контрастные и более размытые. Соавторы: Суббот А.М., Бурсов А.И. English: Monolayer culture of human corneal limbal epithelial cells. This is a BSE-image of a lanthanoid-stained sample taken with EVO LS10 scanning electron microscope (Zeiss, Germany) without prior fixation, nor dehydration, nor sputtering (a method developed by BioREE, Russia, with support from the Research Institute of Eye Diseases, Russia). Nuclei, nucleoli, and extracellular matrix components are clearly visible. Cell borders, intercellular contacts in particular, are very bright because of having taken up the rare earth based contrast agent. One can say that a sample thus prepared resembles a block of ice with air bubbles in it – the deeper an object lies, the lower contrast it has and the more diffused it appears. Coauthors: A.M. Subbot, A.I. Bursov |
| Date | |
| Source | Own work |
| Author | Ivan A. Novikov |
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This image was uploaded as part of European Science Photo Competition 2015. |
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| current | 11:15, 11 November 2015 | | 3,072 × 2,304 (4.74 MB) | Novikov76 (talk | contribs) | User created page with UploadWizard |
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