File:Minimum Evolution-Tree Aleurobotrys and related Taxa.svg

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Figure. Figure. Evolutionary relationships of Aleurobotrys and related Taxa
The evolutionary history was inferred using the Minimum Evolution method [1]. The optimal tree with the sum of branch length = 0.24349473 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches [2]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree.(For all > 50%) The evolutionary distances were computed using the Kimura 2-parameter method [3] and are in the units of the number of base substitutions per site. The ME tree was searched using the Close-Neighbor-Interchange (CNI) algorithm [4] at a search level of 2. The Neighbor-joining algorithm [5] was used to generate the initial tree. The analysis involved 20 nucleotide sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 1171 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [6].


1. Rzhetsky A. and Nei M. (1992). A simple method for estimating and testing minimum evolution trees. Molecular Biology and Evolution 9:945-967.
2. Felsenstein J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783-791.
3. Kimura M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111-120.
4. Nei M. and Kumar S. (2000). Molecular Evolution and Phylogenetics. Oxford University Press, New York. 5. Saitou N. and Nei M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
6. Tamura K., Stecher G., Peterson D., Filipski A., and Kumar S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution30: 2725-2729.

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