File:Pulse-chase analysis of auxin signal transduction in Arabidopsis thaliana wildtype and mutant.png
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DescriptionPulse-chase analysis of auxin signal transduction in Arabidopsis thaliana wildtype and mutant.png |
English: Wild-type Arabidopsis thaliana (left) and the axr2-1 mutant (right). axr2-1 is a dominant gain-of-function mutation in an Aux/IAA gene that confers reduced auxin response. The mutant axr2-1 protein constitutively represses auxin response because it cannot be targeted for proteolysis by the SCFTIR1 ubiquitin ligase. The effect of the mutation on AXR2 stability is shown in a pulse-chase experiment (inset). Wild-type and axr2-1 seedlings were labeled with 35S-methionine and AXR2/axr2-1 protein was immunoprecipitated either immediately after the labeling period (t = 0) or following a 15-minute chase with unlabeled methionine (t = 15). |
Date | |
Source | Gray, W. M. (2004). "Hormonal Regulation of Plant Growth and Development". PLoS Biology 2 (9): e311. DOI:10.1371/journal.pbio.0020311. PMID 15367944. PMC: 516799. |
Author | William M. Gray |
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