File:The perfect sphere.jpg

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Tobacco protoplast

Summary[edit]

Description
English: Protoplasts are plant cells deprived of the cell wall thanks to the combined action of the cellulase and pectinase enzymes. The protoplast in the photo, isolated from the foliar mesophyll of a tobacco plant (Nicotiana tabacum), takes on a spherical shape being devoid of the rigid wall but is still able to perform normal metabolic functions. Particular attention must be paid to the osmotic pressure of the culture medium which must be isotonic with the cellular environment to avoid protoplast rupture. Protoplasts can be manipulated with different cellular and molecular engineering techniques. Through their fusion, for example, it is possible to obtain the so-called somatic and c-hybrid hybrids that are used in genetic improvement techniques. (Differential interference contrast optical microscope, 400 x).
Date
Source Own work
Author Massimo Vischi

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Date/TimeThumbnailDimensionsUserComment
current15:11, 12 December 2019Thumbnail for version as of 15:11, 12 December 20194,724 × 2,362 (1.99 MB)Visma21 (talk | contribs)User created page with UploadWizard

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