File:Western blot 2451.jpg

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Français : Le principe de l’immuno-détection couplé à une ECL. Exemple pour le cas d'une détection de la streptavidine. Le principe est de fixer un anticorps primaire sur notre protéine attachée à une membrane de nitrocellulose, en l’occurrence l’anticorps se fixe sur la queue des six histidines (ajout artificiel). Un anticorps secondaire se fixe au premier. Celui-ci possède une peroxydase qui permettra au luminol d’émettre de la lumière lors de sa catalyse.
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Author George Chèvre

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current17:45, 20 December 2011Thumbnail for version as of 17:45, 20 December 20112,592 × 3,872 (765 KB)George Chèvre (talk | contribs)

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