File:Hypothesized evolution of a truffle lineage.png

English: In this scenario the habitat of an epigeous species with 8-spored, uniseriate asci becomes more arid (A). Selection for reduced water loss results in an enclosed truffle form that has hymenium-lined chambers and asci that are shorter and more clavate in form (B). The ability to forcibly discharge spores is lost and selection for other means of spore dispersal intensifies, leading to spore dispersal through animal mycophagy. Continued selection results in a truffle species that fruits belowground and has a solid gleba stuffed with spherical asci packed with irregular numbers of spores (C).