File:Summary of SAGE.svg
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[llamk'apuy]| T'iktuna |
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
| P'unchaw | |
| Pukyu | Kikinpa rurasqan |
| Ruraq | Thomas Shafee |
| Huk musuqchasqakuna |
Also in series:
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Saqillaspa
[llamk'apuy]
Ñuqaqa, kay rurasqaypa iskaychay hayñiyuq kaqnin, kaywan rurasqayta uyaychachkanim kay qatiq saqillaywan:
Este archivo está disponible bajo la licencia Creative Commons Atribución 4.0 Internacional.
- Qispilla rurarillay:
- rakinakuy – iskaychay, mast'ariy, maymanpas kachay kay rurasqata
- musuqmanta chaqruy – rurasqata tupayay
- Kay phatakuna tukuptinqa:
- Ruraqpa sutinta willay – Kay rurasqataqa ruraqninpa icha saqillaqninpa sut'ichasqan hina unanchanaykim (ichataq amapuni kay hinachu, pay q'imisunkiman icha rurayniykita q'iminman rikch'akunman).
- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
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P'unchaw/pacha nisqapi ñit'iy chaypacha willañiqi kachkasqata qhawanaykipaq.
| P'unchaw/Pacha | Uchuylla rikchacha | Chhikanyachikuqkuna | Ruraq | Willapuy | |
|---|---|---|---|---|---|
| kunan | 12:21 13 phi 2017 | | 938 × 756 (180 kB) | Evolution and evolvability (rimanakuy | llamk'apusqakuna) | text as paths to avoid artefacts |
| 10:48 13 phi 2017 |  | 938 × 756 (104 kB) | Evolution and evolvability (rimanakuy | llamk'apusqakuna) | update sequence text to 'sans-serif' open font for more robust rendering | |
| 05:27 7 ukt 2016 |  | 938 × 756 (104 kB) | Evolution and evolvability (rimanakuy | llamk'apusqakuna) | ce | |
| 05:23 7 ukt 2016 |  | 938 × 756 (102 kB) | Evolution and evolvability (rimanakuy | llamk'apusqakuna) | ce | |
| 05:16 7 ukt 2016 |  | 938 × 756 (104 kB) | Evolution and evolvability (rimanakuy | llamk'apusqakuna) | digitag-->tag | |
| 05:08 5 ukt 2016 |  | 1020 × 821 (106 kB) | Evolution and evolvability (rimanakuy | llamk'apusqakuna) | User created page with UploadWizard |
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