File:A-dynamin-1--dynamin-3--and-clathrin-independent-pathway-of-synaptic-vesicle-recycling-mediated-by-elife01621f009.jpg

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English: Neuronal cultures were incubated overnight in TTX and then exposed to 90 s stimulation in high K+ and recovered for 3 and 10 min. (A) Representative examples of Dyn3 KO (control) and Dyn1/3 DKO synapses. (B) Gallery of coated buds on vacuole-like structures: note the connection to the plasma membrane in B4 (red arrows). (C) CCPs on PM invaginations at Dyn1/3 DKO synapses. C1, EM micrograph; C2-C3, 3-D models from tomograms illustrating CCP trees; C4, CCPs (white arrowheads) on a dilated PM invagination. A white bracket and an asterisk indicates the portion of the PM directly apposed to the postsynaptic membrane (red) which is visible through the transparent green presynaptic plasma membrane. (D) Constricted bulk endosomes. Serial images D3-2 to D3-4 show constrictions and serial images D4-2 to D4-4 show constrictions and a fenestration. Red arrows point to membrane discontinuities. Scale bars: A, 250 nm; B and C1, 3 and 4, 100 nm; C2, 50 nm; D, 50 nm (3D), 20 nm (single images).
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Source Image file from Wu Y, O'Toole E, Girard M, Ritter B, Messa M, Liu X, McPherson P, Ferguson S, De Camilli P (2014). "A dynamin 1-, dynamin 3- and clathrin-independent pathway of synaptic vesicle recycling mediated by bulk endocytosis". eLife. DOI:10.7554/eLife.01621. PMID 24963135. PMC: 4107917.
Author Wu Y, O'Toole E, Girard M, Ritter B, Messa M, Liu X, McPherson P, Ferguson S, De Camilli P
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current22:34, 30 September 2014Thumbnail for version as of 22:34, 30 September 2014701 × 1,026 (274 KB)Recitation-bot (talk | contribs)Automatic upload of media from: doi:10.7554/eLife.01621

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