File:Examination of the urine; a manual for students and practitioners (1909) (14590646030).jpg

Identifier: examinationofu00saxe (find matches)
Title: Examination of the urine; a manual for students and practitioners
Year: 1909 (1900s)
Authors: Saxe, George Alexander De Santos, 1876-1911
Subjects: Urine
Publisher: Philadelphia and London, W. B. Saunders Company
Contributing Library: The Library of Congress
Digitizing Sponsor: The Library of Congress

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-green and then a brilliant blue contact-ring isproduced when blood-pigments are present. Pus also gives this ring, butin this case the ring disappears on heating the mixture. The blood ring,however, stays. Before using this test an alkaline urine must be madeacid. This test is very delicate. Benzidin Test.—A test similar to the guaiacum test, but still moredelicate. The reagent consists of J cc. of a fresh solution of Merckspure benzidin in glacial acetic acid and 2 or 3 cc. of 3 per cent, hydro-gen peroxid. To 10 cc. of urine add 0.5 to 1.0 cc. of glacial acetic acid,and shake well. Then add one-third volume of ether and shake thor-oughly. Allow to stand a little while and add 5 to 10 drops of absolutealcohol. Shake gently and pipet off the cleared ethereal layer. Thelatter is added to the benzidin reagent in another test-tube and the mix-ture is well-shaken. If small amounts of blood are present, a green colordevelops; if larger amounts, a blue color. The reaction is about twenty
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Fig. 28.—Spectra: 1 a, Oxyhemoglobin; &, oxygen-free hemoglobin.2, Methemoglobin : a, in neutral solution; b\ in alkaline solution. 3,Hematin in acid alcoholic solution; 6, in ammoniacal solution; c) reduce4hematin (after Neubauer and Vogel). 196 BLOOD-PIGMENTS 197 times more delicate than Hellers. It can be used both in hematuria andhemoglobinuria, and is not interfered with by pus, sugar, bile, iodide,senna, or rhubarb (Schlesinger and Hoist). Hellers Test.—The earthy phosphates are precipitated from the urineby means of caustic potash solution and gentle heat. The precipitateof earthy phosphates carries with it, as it sinks, the blood coloring-mattersand appears not white, as in normal urine, but blood-red. In alkalineurine the phosphates may be precipitated by a few drops of the magnesiumfluid (see p. 218) on the application of heat. Hellers test is not nearly sodelicate as the above tests. It is open to the objection that it reacts withhematoporphyrin and with certain veg

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