File:Unlocking-Short-Read-Sequencing-for-Metagenomics-pone.0011840.g007.jpg
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English: Mate-reads from the control lane (PhiX174 bacteriophage genome) were used to assess false alignments introduced by the SHERA pipeline. After constructing the composite sequences and filtering, we plotted the difference in length (if any) between a composite fragment and its insert length as predicted by MAQ by mapping the original mate-pairs to the reference.This histogram is a sum of two distributions, the overlapper software's misalignments (a broad gaussian) and a sharp peak of small (1–2 bp) indels. We used a simple and conservative linear model to remove the indels and infer the number of misalignments. |
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| Source | Image file from Rodrigue S, Materna A, Timberlake S, Blackburn M, Malmstrom R, Alm E, Chisholm S (2010). "Unlocking Short Read Sequencing for Metagenomics". PLOS ONE. DOI:10.1371/journal.pone.0011840. PMID 20676378. PMC: 2911387. | |
| Author | Rodrigue S, Materna A, Timberlake S, Blackburn M, Malmstrom R, Alm E, Chisholm S | |
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  This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 21:46, 11 October 2014 | | 430 × 271 (20 KB) | Recitation-bot (talk | contribs) | Automatic upload of media from: doi:10.1371/journal.pone.0011840 |
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