File:Zebra fish U0126 application during a specific time-window decreases ERK phosphorylation specifically.jpeg
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| Description | Zebra fish. U0126 application during a specific time-window decreases ERK phosphorylation specifically. A: Diphospho-Erk staining in the trunk of wt 24 hpf embryos.B: U0126 eliminates ERK1/2 phosphorylation (green) in the trunk and tail of 24 hpf zebrafish embryos. Notice that U0126-treated embryos have been overexposed (can be judged by the autofluorescence of the yolk sac extension) and still fail to show specific staining. C: U0124 does not eliminate the p-Erk staining. D: PD98059 also does not eliminate p-Erk staining when given just below the level of lethal toxicity (25 μM). E: Somewhat weaker but still significant p-Erk staining in embryos treated with U0126 starting at 10 hpf. F: Western-blot analysis demonstrated a slight but incomplete reduction in pErk1/2 levels in 10-somite embryos.G: Stage and dose-dependency of U0126 application. There is a dramatic drop in the percentage of affected embryos when treatment is applied after epiboly is completed. H: Washout experiments reveal the necessity of U0126 to be present until at least the 18 somite stage to produce the full phenotype. |
| Date | |
| Source | https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-8-42#Fig1 https://bmcdevbiol.biomedcentral.com/articles/10.1186/1471-213X-8-42 Hawkins, T.A., Cavodeassi, F., Erdélyi, F. et al. The small molecule Mek1/2 inhibitor U0126 disrupts the chordamesoderm to notochord transition in zebrafish. BMC Dev Biol 8, 42 (2008). |
| Author | Hawkins, T.A., Cavodeassi, F., Erdélyi, F. et al. |
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| current | 00:29, 25 April 2024 | | 1,826 × 3,189 (1.3 MB) | Rasbak (talk | contribs) | {{Information |description=Zebra fish. U0126 application during a specific time-window decreases ERK phosphorylation specifically. A: Diphospho-Erk staining in the trunk of wt 24 hpf embryos.B: U0126 eliminates ERK1/2 phosphorylation (green) in the trunk and tail of 24 hpf zebrafish embryos. Notice that U0126-treated embryos have been overexposed (can be judged by the autofluorescence of the yolk sac extension) and still fail to show specific staining. C: U0124 does not eliminate the p-Erk st... |
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