File talk:DNA replication en.svg

a new image was created to address this issues have a look at

-LadyofHats (talk) 21:26, 2 July 2019 (UTC)

 

colours in helix are swapped :))

There is a lot of outdated or just plain wrong information on this graphic. It has been established that pol delta is the lagging strand polymerase, pol alpha is the primase, and pol epsilon is the leading strand polymerase. There are much better models of the replication fork available.

I believe the Ligase position should be prior to the polymerase.

The article says DNA-ligase is the last molecule to act on the DNA, meaning it should be farthest left on the diagram. However, the DNA-polymerase appears to the left of the DNA-ligase. Also, the DNA-polymerases are switched in the diagram, as another contributor has pointed out in another edit.

I am NOT educated at all, and especially in advanced molecular chemistry. However, if a person simply reads the article and reviews the diagram, one will notice that they are mutually conflicte.! —Preceding unsigned comment was added by 174.57.76.207 (talk) (UTC)

Synthesis goes 5' to 3' on the strand being fabricated, so ligase is after polymerase. So the diagram is correct. It would be more clear if the lagging strand had an arrow like the leading strand does. Dmyersturnbull (talk) 03:48, 28 January 2013 (UTC)

The diagram is incorrect. Polymerase must detach before ligase can bind at the 3' end of the Okazaki fragment to form the final phosphodiester bond and a completed double helix. Polymerase then reattaches at a more 5' region of the lagging strand, close to helicase, where an RNA primer has bound, acting as the initiation site for the polymerase to bind and then run 5' to 3' filling in complimentary base pairs until it reaches the previous Okazaki fragment. And so the cycle repeats. These facts are not in dispute - the problem with the diagram is that it is attempting to describe too many ideas simultaneously. Polymerase does its job first, followed by ligase which completes the job. This sequence of events is what the diagram is trying to illustrate by putting the polymerase to the left of the ligase. However, this is technically incorrect, because polymerase has to be removed before ligase can bind. As illustrated, the polymerase is attached to a completed double strand on the left, which is impossible, and ligase is filling in the backbone to the right. The reality is that the ligase would be working on the backbone at the 3' end of the oldest Okazaki fragment. The polymerase meanwhile will be to the right of the ligase building the newer Okazaki fragment closer to the helicase, filling in base pairs 5' to 3' i.e. heading from right to left heading toward the already bound ligase molecule illustrated. Thus, as illustrated the diagram is incorrect, because polymerase cannot bind to double stranded DNA as illustrated and would be bound to the newer Okazaki fragments to the right of the ligase User:drsusan1968.

I'm high school student of Republic of Korea. I also think this diagram is incorrect. In my textbook, DNA polymerase is more close to helicase. Not only that point but also DNA ligase lie in the diagram's Polymerase's position. So i thought like that ♠♠♠

I would join the chorus of those who believe the diagram to be incorrect, and I completely agree with Dr Susan. The confusion certainly also lies in the simplification that the lagging strand is unfolded. In reality the lagging strand is folded so that the bound DNA polymerase follows the helicase just as the DNA polymerase bound to the leading strand follows the helicase of the replication fork (figure 2b here might be easier to understand). If there are other illustrations here on commons that resemble the one that I've linked to, I would be happy to be notified. --Masz (talk) 18:44, 23 December 2016 (UTC)

What now?

Is this stilla problem? Because then somebody should fix it ASAP! --Metalindustrien (talk) 05:23, 15 July 2016 (UTC)

The problems critics are voicing are at least partly due to having to represent a 3 dimensional structure changing over a period of time in a two dimensional static image. Also, molecules such as ligases can potentially operate repeatedly at different stages during the process though may not always do so. Research may describe what a typical process probably looks like but it is rarely definitive of real life. This diagram is definitely a great representation to aid understanding. The text of the articles it is included in obviously goes into the detail and the research it is derived from if points need to be clarified. If the two dimensional snapshot in time can be improved I'm sure someone will eventually do it but that is true of all images on Wikipedia. I'm definitely against deletion. Tgru001 (talk) 23:12, 8 February 2023 (UTC)