File:Радиальная бумажная хроматограмма БАС спиртового извлечении из травы горца птичьего.jpg
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Captions
Captions
Radial paper chromatogram of alcohol extract BAC from knotweed herb in UV light (λ=365 nm) after treatment with an 6% alcohol solution of aluminum chloride
Summary
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| Description |
Русский: Диск хроматографической бумаги (неподвижная фаза, НФ) перфорируют по центру. В равном удалении от отверстия, точечно наносят капилляром 2 мкл спиртового извлечения из травы горца птичьего и 0,05% спиртовые растворы стандартных образцов флавоноидов, в отверстие помещают фитиль из хроматографической бумаги, и хроматографируют в камере с 15% раствором кислоты уксусной (подвижная фаза, ПФ). В процессе хроматографирования соединения спиртового извлечения и стандартных образцов флавоноидов под действием потока ПФ и за счет капиллярных сил перемещаются по НФ, и имея разное сродство к фазам, в определенный момент сорбируются на НФ и перестают передвигаться дальше. Таким образом весь комплекс соединений извлечения разделяется в виде зон, имеющих форму дуг, специфическую окраску флуоресценции и значение Rf-фактора (отношение расстояний пройденного соединением к пройденному подвижной фазой), соответствующих аналогичным показателям стандартных образцов.
English: A disk of chromatographic paper (stationary phase, SF) is perforated in the center. At an equal distance from the hole, 2 µl of an alcohol extract from the knotgrass herb and 0.05% alcohol solutions of reference samples of flavonoids are dotted with a capillary, a wick of chromatographic paper is placed in the hole, and chromatography is performed in a chamber with a 15% solution of acetic acid (mobile phase, MF). In the process of chromatography, the compounds of the alcohol extract and reference samples of flavonoids move along the SF under the action of the MF flow and due to capillary forces, and having different affinities to the phases, at a certain moment are sorbed on the SF and stop moving further. Thus, the entire complex of extract compounds is separated in the form of arc-shaped zones with specific fluorescence color and Rf-factor value (the ratio of the distances travelled by compounds and by the MF), corresponding to similar indicators of reference samples. English: A disk of chromatographic paper (stationary phase, SF) is perforated in the center. At an equal distance from the hole, 2 µl of an alcohol extract from the knotgrass herb and 0.05% alcohol solutions of reference samples of flavonoids are dotted with a capillary, a wick of chromatographic paper is placed in the hole, and chromatography is performed in a chamber with a 15% solution of acetic acid (mobile phase, MF). In the process of chromatography, the compounds of the alcohol extract and reference samples of flavonoids move along the SF under the action of the MF flow and due to capillary forces, and having different affinities to the phases, at a certain moment are sorbed on the SF and stop moving further. Thus, the entire complex of extract compounds is separated in the form of arc-shaped zones with specific fluorescence color and Rf-factor value (the ratio of the distances travelled by compounds and by the MF), corresponding to similar indicators of reference samples. |
| Date | |
| Source | Own work |
| Author | Георгий Родионович Кутателадзе |
Licensing
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I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution 4.0 International license.
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This image was uploaded as part of Russian Science Photo Competition 2023.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 09:28, 14 May 2023 | | 3,430 × 3,452 (3.19 MB) | Georgii Kutateladze (talk | contribs) | Uploaded own work with UploadWizard |
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