File:2022.12.001.gr3 lrg.jpg

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English: EcCIEDL933 produces small capsids

(A) Electron microscopy analyses of different MC-induced HK106 lysogenic strains (wildtype [WT], terL mutant [gp01∗] or capsid mutant [gp05∗]) in presence or absence of EcCIEDL933 (wildtype [WT], terL mutant [1794∗] or capsid mutant [1786∗]). Different fields are shown, containing HK106 phage particles (top images), EcCIEDL933 particles (bottom images), or both (bottom left; PICI particles highlighted with white arrows). EcCIEDL933 particles have smaller heads. Images were collected using a JEOL 1200 TEM microscope with 12K magnification. Scale bars represent 200 nm.
(B) Lysogenic strains carrying the WT or the terL mutant (gp01∗) HK106 prophage, in presence or absence of EcCIEDL933, were MC induced; the DNA was extracted from the purified infective particles and resolved on a 0.7% agarose gel, GelRed stained. The size of phage HK106 is 41,468 bp, while EcCIEDL933 has a size of 15,471 bp.

(C) Southern blot of the purified DNA shown in (B) using either phage HK106- or EcCIEDL933-specific probes. First line contains the Southern blot molecular marker (DNA molecular weight marker VII; Roche).
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Source A widespread family of phage-inducible chromosomal islands only steals bacteriophage tails to spread in nature. Volume 31, Issue 1, P69-82.e5; doi:10.1016/j.chom.2022.12.001.
Author Nasser Alqurainy, Laura Miguel-Romero, Jorge Moura de Sousa, John Chen, Eduardo P. C. Rocha, Alfred Fillol-Salom, José R. Penadés
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