File:Didesoxymethode.svg

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Description
Deutsch: Prinzip der DNA-Sequenzierung nach der Didesoxy-Methode. dNTP ist die allgemeine Abkürzung für ein Nucleotid und kann für dATP, dCTP, dGTP oder dTTP stehen. ddNTPs sind die entsprechenden Didesoxy-Varianten der dNTPs. Der Einbau eines ddNTPs führt zum Abbruch der Polymerisationsreaktion. Die blauen Punkte am 5'-Ende des Primers stellen eine Markierung dar (z.B. eine fluoreszierende Gruppe), mittels der die Syntheseprodukte später im Gel sichtbar gemacht werden können. Alternativ lassen sich auch radioaktiv markierte Nucleotide zur Polymerisationsreaktion einsetzen.
Date
Source Christoph Goemans
Author Christoph Goemans

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GNU head Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License.
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  • 2009-07-13 12:34 Mæx 620×455× (184926 bytes) {{Information |Beschreibung = Prinzip der DNA-Sequenzierung nach der Didesoxy-Methode. dNTP ist die allgemeine Abkürzung für ein Nucleotid und kann für dATP, dCTP, dGTP oder dTTP stehen. ddNTPs sind die entsprechenden Didesoxy-Varianten der dNTPs. Der

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current14:26, 20 May 2010Thumbnail for version as of 14:26, 20 May 2010620 × 455 (181 KB)File Upload Bot (Magnus Manske) (talk | contribs) {{BotMoveToCommons|de.wikipedia|year={{subst:CURRENTYEAR}}|month={{subst:CURRENTMONTHNAME}}|day={{subst:CURRENTDAY}}}} {{Information |Description={{de|Prinzip der DNA-Sequenzierung nach der Didesoxy-Methode. dNTP ist die allgemeine Abkürzung für ein Nu

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