File:Effect of RNAi-mediated knockdown of SchgrETHR or SchgrETHpre on the last immature and adult moult of S. gregaria.jpg

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English: (A) Percentage of instars arrested at the last immature and adult moult (N = 17–19). Locusts were injected on day 1 and 4 of the fourth instar stage, as well as on day 1, 3 and 5 of the fifth instar stage with 400 ng of dsRNA against GFP (control), SchgrETHR or SchgrETHpre. (B) Phenotypes correlated with the arrested moult at the expected timing of ecdysis. Top: RNAi phenotype (weakened and died); Middle and bottom: respectively control fifth instar and adult locusts. (C) Percentage of instars arrested at the last immature moult (N = 12–15). Locusts were injected on day 0, 2 and 4 of the fourth instar stage with 400 ng of dsRNA against GFP (control) or SchgrETHpre (dsSchgrETHpre and rescue). To rescue the knockdown of SchgrETHpre, locusts were injected with 10 ng of ETH1 and ETH2 once a day from day 0 till day 3 and with 100 ng of both peptides twice a day from day 4 until the day they moulted or died. Control and dsSchgrETHpre locusts were injected with Schistocerca Ringer according to the same injection scheme. Significant differences (p < 0.001 and p < 0.0001) are indicated by asterisks (*** and **** respectively) (Fisher’s exact test).
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Source https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394484/
Author Cynthia Lenaerts, Dorien Cools, Rik Verdonck, Lina Verbakel, Jozef Vanden Broeck and Elisabeth Marchala

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current15:38, 4 April 2024Thumbnail for version as of 15:38, 4 April 2024697 × 928 (203 KB)VGPaleontologist (talk | contribs)Uploaded a work by Cynthia Lenaerts, Dorien Cools, Rik Verdonck, Lina Verbakel, Jozef Vanden Broeck and Elisabeth Marchala from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394484/ with UploadWizard

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