File:Facilitating and verifying an isochoric vitrification process for cryopreservation and revival of corals.webp

English: "a Custom-designed isochoric chamber rated for 400 MPa/−200 °C. The chamber body is built of aluminum 7075-T651 for its combination of excellent thermal and mechanical properties, and the threaded adapter is built of 316 stainless steel. The adapter possesses a significantly lower temperature coefficient of thermal expansion compared to Al-7075, thus producing an advantageous thermal shrink-seal upon cooling. b Pressure–temperature–time (PTT) trace for successful vitrification, as indicated by the absence of a jump in pressure (n = 10 independent trials). c, d PTT traces for slower cooling (c) and warming (d) rates, each of which produce detectable ice nucleation and the according pressurization (n = 10 independent trials, each). Each vitrification trial reflected in (b–d) employed a fresh sample of CVS1 solution. Solid lines in (b–d) represent the mean PTT trace across trials, and shaded error bands represent the full range of results. Source data for (b–d) are provided as a Source data file."