File:Fpls-02-00005-g004.png

English: Conserved phosphorylation sites of interest. The conservation of observed phosphorylation sites is detailed by frequency plots. Red asterisks are placed above positions in which phosphorylated peptides were observed, but the specific residue phosphorylated was ambiguous. Black asterisks are place above unambiguous phosphorylation. A region near the catalytic sites has strongly conserved serines/threonines in the primary CesAs and the moss and lycophyte sequences (A). Serines/threonines observed to be phosphorylated in CesA3 are strongly conserved and aligned to a strongly conserved serine/threonine in CesA1 and the moss and lycophyte sequences (B). A phosphorylated region shows strong conservation within the CesA1 family is also found to have several phosphorylated serines/threonines in the CesA3 family, but only in eudicots (labeled 3D), possibly indicating phosphorylation here is a recent adaptation in eudicot CesA3s (C).