File:Herpes-Simplex-Virus-Dances-with-Amyloid-Precursor-Protein-while-Exiting-the-Cell-pone.0017966.s010.ogv
Материал из Викисклада, хранилища свободных медиафайлов
Перейти к навигации
Перейти к поиску
Нет версии с бо́льшим разрешением.
Herpes-Simplex-Virus-Dances-with-Amyloid-Precursor-Protein-while-Exiting-the-Cell-pone.0017966.s010.ogv (Ogg-видеофайл Theora. Длительность: 23 с. 598 × 314 пкс, битрейт: 113 Кбит/с, размер файла: 313 КБ)
Сведения о файле
Структурированные данные
Краткие подписи
Краткие подписи
Добавьте однострочное описание того, что собой представляет этот файл
Краткое описание
[править]ОписаниеHerpes-Simplex-Virus-Dances-with-Amyloid-Precursor-Protein-while-Exiting-the-Cell-pone.0017966.s010.ogv |
English: APP stains gEnull viral particles. This sequence shows the 3D rendering of a z-stack of the cell shown in Figure 3 that was infected with gE null virus (green) and stained for APP (red) and DAPI (blue).
Quantitative validation of infection protocol. (A) Distribution of viral particles in infected cells. Cells infected with VP26-HSV1, incubated for 7–9 hr p.i., fixed and stained for DAPI as in Figure 1. Numbers of cells in each of five categories representing distribution of viral particles as diagrammed were counted from digital images taken at random from 3 different experiments. After constitutive infection (96.2+/− 6.7% infected, top row) when virus remains in the medium throughout incubation, cells displayed GFP-labeled particles in all 5 distribution categories, including 29% that display >10 cytoplasmic particles with no evidence of nuclear GFP synthesis. These GFP particles thus must represent in-coming GFP-labeled virus. In parallel cultures infected for 1 hr with our protocol (87.5 +/− 24.4% infected, lower row), no cells were found with >10 cytoplasmic GFP-particles in the absence of nuclear GFP (boxed column), and a higher percentage of cells were found with only nuclear GFP, or both nuclear GFP and cytoplasmic GFP particles (lower row). Thus, after synchronized infection only small numbers of VP26-GFP particles are found in the cytoplasm of cells not expressing viral genes as evidenced by absence of viral-encoded VP26-GFP in the nucleus. (B) Average number of VP26-GFP labeled viral particles in the cytoplasm after synchronization. To determine the average number of incoming viral particles in the cytoplasm at later stages of infection, we counted particles at two time points (6.5–7 hr and 9 hr) after synchronized infection. At 6.5–7 hr p.i., the two predominant patterns in productively infected cells were: (1) few cytoplasmic VP26-GFP-particles whether or not there was nuclear GFP (3+/−3.0 and 3+/−2.3 cytoplasmic particles respectively); or (2) many cytoplasmic particles in cells with strong nuclear GFP (65+/−5 cytoplasmic particles). Less than 3% of cells displayed other distributions, and none had many cytoplasmic particles without nuclear GFP. At 9 hr p.i. all infected cells had strong nuclear GFP and many particles in the cytoplasm. Experiments were done in triplicate and counts (16,300 GFP particles) were made on at least three different coverslips for each condition for no less than 10 microscopic fields per coverslip. |
||
Дата | |||
Источник | Movie S1 from Cheng S, Ferland P, Webster P, Bearer E (2011). "Herpes Simplex Virus Dances with Amyloid Precursor Protein while Exiting the Cell". PLOS ONE. DOI:10.1371/journal.pone.0017966. PMID 21483850. PMC: 3069030. | ||
Автор | Cheng S, Ferland P, Webster P, Bearer E | ||
Права (Повторное использование этого файла) |
|
||
Provenance InfoField |
|
История файла
Нажмите на дату/время, чтобы увидеть версию файла от того времени.
Дата/время | Миниатюра | Размеры | Участник | Примечание | |
---|---|---|---|---|---|
текущий | 14:47, 31 октября 2012 | 23 с, 598 × 314 (313 КБ) | Open Access Media Importer Bot (обсуждение | вклад) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
Вы не можете перезаписать этот файл.
Использование файла
Нет страниц, использующих этот файл.
Статус декодирования
Обновить статус декодированияМетаданные
Файл содержит дополнительные данные, обычно добавляемые цифровыми камерами или сканерами. Если файл после создания редактировался, то некоторые параметры могут не соответствовать текущему изображению.
Автор | Cheng S, Ferland P, Webster P, Bearer E |
---|---|
Условия использования | http://creativecommons.org/licenses/by/3.0/ |
Название изображения | APP stains gEnull viral particles. This sequence shows the 3D rendering of a z-stack of the cell shown in Figure 3 that was infected with gE null virus (green) and stained for APP (red) and DAPI (blue). |
Программное обеспечение | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Дата и время оцифровки | 2011 |
Категории:
- Amyloid beta-protein precursor
- Western blot
- Capsid proteins
- Fluorescent antibody technique
- Videos of green fluorescent proteins
- Microscopic images of cell nucleus stained with DAPI in HeLa cells
- Videos of cell nucleus in HeLa cells
- Videos of viral infections in HeLa cells
- Confocal microscopic movies from Open Access Journals
- Videos of electron microscopes
- Small interfering RNA
- Simplexvirus
- Vero cells
- Media from PLOS ONE