File:PBR322 plasmid.gif

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Description
Русский: Карта плазмиды pBR 322, применяемой для клонирования участков чужеродной ДНК в клетках бактерии E. coli.


Cпециально создана Франциско Болваром и Раймондом Родригесом с целью клонирования ДНК. Представляет собой циклический фрагмент ДНК длиной 4362 нуклеотидных пары. Плазмида содержит ген устойчивости к тетрациклину tet, взятый из естественной плазмиды pSC 101; ген устойчивости к ампициллину amp, взятый из транспозона Tn3; и участок начала репликации ori, заимствованный из плазмиды pMB 1. Плазмида содержит также сайты рестрикции Pst I, Bam HI и Sal I, причём первый находится в гене amp, а два остальных – в гене tet.

Айала Ф., Кайгер Дж. Современная генетика: В 3-х т. Т. 1. Пер. с англ.: — М.: Мир, 1987. — 295 с., ил.
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Author AdmiralHood (talk) 13:19, 5 November 2010 (UTC)

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current13:19, 5 November 2010Thumbnail for version as of 13:19, 5 November 2010794 × 729 (23 KB)AdmiralHood (talk | contribs){{Information |Description={{ru|1=Карта плазмиды pBR 322, применяемой для клонирования участков чужеродной ДНК в клетках бактерии ''E. coli''. Cпециально создана

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