File:Summary of SAGE.svg
From Wikimedia Commons
Jump to navigation
Jump to search
Size of this PNG preview of this SVG file: 744 × 600 pixels. Other resolutions: 298 × 240 pixels | 596 × 480 pixels | 953 × 768 pixels | 1,271 × 1,024 pixels | 2,541 × 2,048 pixels | 938 × 756 pixels.
Original file (SVG file, nominally 938 × 756 pixels, file size: 180 KB)
File information
Structured data
Captions
Summary
[edit]DescriptionSummary of SAGE.svg |
English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1] |
Date | |
Source | Own work |
Author | Thomas Shafee |
Other versions |
Also in series:
|
Licensing
[edit]I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 12:21, 13 February 2017 | 938 × 756 (180 KB) | Evolution and evolvability (talk | contribs) | text as paths to avoid artefacts | |
10:48, 13 February 2017 | 938 × 756 (104 KB) | Evolution and evolvability (talk | contribs) | update sequence text to 'sans-serif' open font for more robust rendering | ||
05:27, 7 October 2016 | 938 × 756 (104 KB) | Evolution and evolvability (talk | contribs) | ce | ||
05:23, 7 October 2016 | 938 × 756 (102 KB) | Evolution and evolvability (talk | contribs) | ce | ||
05:16, 7 October 2016 | 938 × 756 (104 KB) | Evolution and evolvability (talk | contribs) | digitag-->tag | ||
05:08, 5 October 2016 | 1,020 × 821 (106 KB) | Evolution and evolvability (talk | contribs) | User created page with UploadWizard |
You cannot overwrite this file.
File usage
The following 5 pages use this file:
Global file usage
The following other wikis use this file:
- Usage on ar.wikipedia.org
- Usage on en.wikipedia.org
- Usage on en.wikiversity.org
- Usage on es.wikipedia.org
- Usage on meta.wikimedia.org
- Usage on ru.wikipedia.org
Metadata
This file contains additional information, probably added from the digital camera or scanner used to create or digitize it.
If the file has been modified from its original state, some details may not fully reflect the modified file.
Width | 938.29999 |
---|---|
Height | 755.59998 |
Structured data
Items portrayed in this file
depicts English
creator English
Some value without a Wikidata item
copyright status English
copyrighted English
copyright license English
source of file English
original creation by uploader English
inception English
5 October 2016
Hidden categories: