File:Zambian wheat blast isolates are susceptible to strobilurin fungicides but at risk from resistance development and can mate with prevailing finger millet blast isolates.png

English: "(A) The tree describes, based on nuclear SNPs, the phylogenetic relationship among wheat-infecting blast isolates belonging to three clonal lineages: B71, PY0925, and P29. The tree was rooted in the midpoint. The colored dots next to each isolate label represent the resistant-type allele of the mitochondrially encoded cyt b gene associated with a susceptible or resistant predicted phenotype as shown in the inset. (B, C) 4 × 106 spores of the Zambian strain ZMW20-14 were exposed to 100 μg ml1 azoxystrobin to obtain an azoxystrobin-resistant strain with the G143A mutation (SR1). This strain, the rice infecting strain Guy11 (G11), and the wheat-infecting strains numbered 1–31, respectively, BTBa-B1, BTBA-B2, BTGS6e, BTGS6f, BTGS6g, BTGS6h, BTGP1-b, BTMP-S13-1, PY6047, BTJP4-1, BTJP4-2, BTJP4-3, BTJP4-5, BTJP4-6, BTJP4-9, BTJP4-11, BTJP4-12, BTJP4-15, BTJP4-16, BTJP4-18, ZMW18-06, ZMW20-07, ZMW18-08, ZMW19-09, ZMW18-10, ZMW18-11, ZMW19-13, ZMW20-14, ZMW20-15, ZMW20-16, and ZMW19-17 were grown for 4 days at 25°C on CM in the absence (A) and presence (B) of azoxystrobin indicating that all the Zambian and Bangladeshi isolates have the “strobilurin susceptible” genotype as anticipated by their CYTB sequences. (D) Zambian isolate ZMW20-7 (MAT-1-2) successfully produced perithecia when crossed with a finger millet blast isolate K1 (MAT-1-1) but (E) ZMW20-7 was unable to produce perithecia when crossed with a finger millet blast isolate T26 of the same mating type (MAT-1-2). Photos taken by the authors. The data underlying this figure can be found in https://doi.org/10.5281/zenodo.7590238. SNP, single nucleotide polymorphism."