File:Zymografia żelowa.png
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[edit]DescriptionZymografia żelowa.png |
Polski: (a) Zymografia żelowa (IGZ) i (b) zymografia żelowo-transferowa (TGZ) (a) W IGZ substrat jest włączony do żelu SDS-PAGE. Po rozdzieleniu według względnych mas cząsteczkowych żel inkubuje się przy optymalnym pH i temperaturze w celu wywołania reakcji proteolitycznej. Żel jest następnie barwiony, a prążki hydrolityczne są przejrzyste na ciemnym tle. (b) W TGZ próbki najpierw rozdziela się na zwykłym żelu SDS-PAGE. Następnie żel rozdzielający układa się na jednym lub wielu żelach zawierających substrat i białka przenosi się na żele substratu. Te ostatnie są przemywane, inkubowane w celu przeprowadzenia reakcji hydrolitycznej, a następnie barwione. Prążki hydrolityczne są zabarwione. |
Date | |
Source | Vandooren, J., Geurts, N., Martens, E., Van den Steen, P. E., & Opdenakker, G. (2013). Zymography methods for visualizing hydrolytic enzymes. Nature Methods, 10(3), 211–220. doi:10.1038/nmeth.2371 |
Author | Vandooren, J., Geurts, N., Martens, E., Van den Steen, P. E., & Opdenakker, G. |
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current | 19:18, 1 June 2021 | 1,152 × 1,152 (171 KB) | Jeanne Rosins (talk | contribs) | Uploaded a work by Vandooren, J., Geurts, N., Martens, E., Van den Steen, P. E., & Opdenakker, G. from Vandooren, J., Geurts, N., Martens, E., Van den Steen, P. E., & Opdenakker, G. (2013). Zymography methods for visualizing hydrolytic enzymes. Nature Methods, 10(3), 211–220. doi:10.1038/nmeth.2371 with UploadWizard |
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