File:An-electrostatic-mechanism-for-Ca2+-mediated-regulation-of-gap-junction-channels-ncomms9770-s2.ogv

English: Supplementary Movie 1 Ca2+ coordination sites reside at the interface between adjacent subunits, near the entrance to the extracellular gap, where local conformational rearrangements enable Ca2+-chelation. Each of the 12 Ca2+ binding sites is comprised of the G45-O and the E47 carboxylate from one subunit, and the E42 carboxylate of the adjacent subunit. The Ca2+-bound and Ca2+-free structures were superimposed, and the morph conformations tool in Chimera was used to create a conformational trajectory between the two structures (morph model, cyan; Ca2+ atom, yellow; coordinating atoms, sticks).