File:Antigen-Specific-Suppression-and-Immunological-Synapse-Formation-by-Regulatory-T-Cells-Require-the-pone.0073874.s006.ogv
Antigen-Specific-Suppression-and-Immunological-Synapse-Formation-by-Regulatory-T-Cells-Require-the-pone.0073874.s006.ogv (Ogg Theora video file, length 6.1 s, 380 × 380 pixels, 785 kbps, file size: 584 KB)
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DescriptionAntigen-Specific-Suppression-and-Immunological-Synapse-Formation-by-Regulatory-T-Cells-Require-the-pone.0073874.s006.ogv |
English: Two-photon imaging of naïve T cells and Mst1+/+ Treg cells in LN tissues. Time-lapse video of OT-II naïve T cells (blue) and Mst1+/+ OT-II Treg cells (red) interacting with OVA323–339-pulsed DCs (green) in LN tissues. LPS-stimulated, OVA peptide-pulsed BMDCs subcutaneously injected into C57BL/6 mice. After 24 h, brachial LNs were isolated and cut open, followed by direct application of the mixture of CMAC- labeled OT-II naïve T cells and CMTMR-labeled Mst1+/+ or Mst1-/- OT-II Treg cells to the cut LN sliced tissues and time-lapse images were taken by two-photon microscopy as described in Methods. Bars indicate 25µm. |
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Source | Video S4 from Tomiyama T, Ueda Y, Katakai T, Kondo N, Okazaki K, Kinashi T (2013). "Antigen-Specific Suppression and Immunological Synapse Formation by Regulatory T Cells Require the Mst1 Kinase". PLOS ONE. DOI:10.1371/journal.pone.0073874. PMC: 3767606. | ||
Author | Tomiyama T, Ueda Y, Katakai T, Kondo N, Okazaki K, Kinashi T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:48, 14 September 2013 | 6.1 s, 380 × 380 (584 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Tomiyama T, Ueda Y, Katakai T, Kondo N, Okazaki K, Kinashi T |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |