File:Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s007.ogv

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Botulinum-Neurotoxins-A-and-E-Undergo-Retrograde-Axonal-Transport-in-Primary-Motor-Neurons-ppat.1003087.s007.ogv ‎(Ogg Theora video file, length 42 s, 509 × 63 pixels, 67 kbps, file size: 348 KB)

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English: HCE undergoes fast retrograde transport in non acidic organelles. Motor neurons cultures were incubated with fluorescent HCE (green) and Lysotracker (red) for 30 min at 37°C under depolarising conditions (60 mM KCl). Cells were then washed and imaged. HCE is retrogradely transported in organelles lacking Lysotracker. As discussed in the text, some of the carriers containing HCE (green) show a discontinuous transport in the retrograde direction (from left to right), whereas some others undergo anterograde transport. Cell body is out of view on the right. Frames were taken every 4 s and played at 5 frames/s. This movie is a representative example of experiments performed on two independent primary motor neuron cultures, replicated twice.
Date
Source Movie S4 from Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G (2012). "Botulinum Neurotoxins A and E Undergo Retrograde Axonal Transport in Primary Motor Neurons". PLOS Pathogens. DOI:10.1371/journal.ppat.1003087. PMID 23300443. PMC: 3531519.
Author Restani L, Giribaldi F, Manich M, Bercsenyi K, Menendez G, Rossetto O, Caleo M, Schiavo G
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current17:01, 21 January 201342 s, 509 × 63 (348 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 240P 20 kbps Completed 04:16, 20 August 2018 4.0 s
Streaming 240p (VP9) 20 kbps Completed 08:01, 16 December 2023 1.0 s
WebM 360P 27 kbps Completed 22:34, 29 November 2023 1.0 s
Streaming 144p (MJPEG) 81 kbps Completed 06:51, 1 November 2023 1.0 s

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